Heatlyse a volume of horse or sheep blood that is 5% of the total volume of media being prepared very slowly to 56c in a water bath. The medium is often used to observate the forms of haemolysis from pathogenic microorganisms. Chocolate agar, enriched, though an excellent culture medium for gonococci, is not a selective medium and. Chocolate agar contains less agar than other solid media therefore streaking should be done carefully to avoid gouging into the agar quality control after checking for correct ph, colour, depth, and sterility, the following organisms are used to determine the growth performance of the completed medium. Blood agar and chocolate agar are quite similar but they differ in preparation.
Chocolate ii agar gc ii agar with hemoglobin and isovitalex for the isolation and cultivation of fastidious. Chocolate agar with tsa and growth supplements is a medium that supports the special growth requirements hemin and nad needed. There are many more dessert recipes that can be made with agaragar. Neisseria chocolate agar culture chocolate agar choc or chocolate blood agar cba is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. The name is itself derived from the fact that red blood cell rbc lysis gives the medium a chocolate brown color. If you continue browsing the site, you agree to the use of cookies on this website. Chocolate agar intended use remel chocolate agar is a solid medium recommended for use in qualitative procedures for the isolation and cultivation of fastidious organisms, especially neisseria and haemophilus species. Haemophilus are small, pleomorphic, gramnegative coccobacilli.
Chocolate ii agar contains an improved gc agar base, bovine hemoglobin and isovitalex enrichment. Allow the media to solidify and condensation to dry. Chocolate agar is used to isolate and cultivate fastidious microorganisms such as neisseria species and haemophilus species. Links to pubmed are also available for selected references.
The blood is treated to remove fibrin, the clotting. The gc base contains nitrogenous nutrients in the form of casein and meat peptones, phosphate buffer to maintain ph and corn starch, which neutralizes toxic fatty acids that may be present in the agar. This organism is oxidasepositive, and growth is best obtained on chocolate agar or thayer martin agar. Potato dextrose agar is used for the preparation and maintenance of fungal test strains used in the growth promotion test, suitability of the counting methods and negative controls as described in the harmonized uspepjp. Chocolate agar with gc base and growth supplement it supports the growth requirements needed to isolate fastidious organisms. The centrifuged deposit should be inoculated on chocolate agar and incubated in a humidified atmosphere and 10%co 2, and on blood agar incubated aerobically. The fluffy and light lemon pie is set over a sweet chocolate base to cut through the lemony flavor.
Bd chocolate agar gc ii agar with isovitalex bd chocolate. Limitaciones las reacciones hemoliticas de una amplia variedad de microorganismos son diferentes al usar sangre equina respecto al utilizar sangre de carnero, tal es el caso de algunas cepas de estreptococos grupo d, que producen beta hemolisis en el agar suplementado. Chocolate agar choc or chocolate blood agar cba, is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. Blood agar consists of many ingredients but the primary ingredient is the blood, which came from a rabbit or a sheep. It is white and simi translucent sold in the form of strips or powder and used in making jellies,pudding and custards thasleen sheik. Chocolate agar is used for the isolation of fastidious organisms, such as haemophilus influenzae, when incubated at. Bbl chocolate ii agar gc ii agar with hemoglobin and. Thermo scientific chocolate agar medium print share. Chocolate agar w yeast extract, hemin, and vitamin k. An enrichment medium should also be inoculated and one such as. Capitulo 44 formualrio magistral en dermocosmetica. Bd sabouraud gc agar chromagar candida medium biplate. Chocolate agar, enriched, though an excellent culture medium for gonococci, is not a selective medium and therefore when mixed cultures are encountered, growth of contaminants is not restricted which may result in overgrowth of gonococci by bacterial and fungal flora often found in specimens from urethral and cervical sites.
The treatment and control of gonorrhea is complicated by increasing resistance to a variety of antimicrobial agents, including penicillin, tetracycline, and fluoroquinolones. Other products used in the isolation of neisseria gonorrhoeae. Shop online for a wide selection of bd bbl prepared plated media. In addition, if the specimen has been obtained from a neonate among whom e. Chocolate agar base, with the addition of supplements, gives excellent growth of the gonococcus without overgrowth by contaminating organisms. It is treated and water and a number of other ingredients are added to sterilize the solution.
576 810 889 707 127 1109 868 339 861 142 756 1208 632 171 610 458 928 79 1410 998 891 905 1134 454 1059 1480 837 631